The GT Analyst supports detection of:

• Fluorescence intensity or polarization
• Luminescence (luciferase)
• Time-resolved fluorescence energy transfer (TR-FRET)

Typical use of the Analyst GT involves introducing one or more transcriptional reporter into cells and screening for up- or down-regulation of the reporter (e.g. firefly or Renilla luciferase). Another common use is to monitor levels, secretion or degradation of a protein tagged with something like GFP or luciferase.

In either case, controlling for cell number (such as with a ubiquitous read-out of Renilla luciferase) can be critical, as you'll want to distinguish true positive hits from false positives or artifacts that result from a reduction of cell number.

Why would I use the Analyst GT?

The Analyst GT provides rapid, fully automated fluorescence or luminescence detection for standard plate-reader screens.

Why would I not use the Analyst GT?

If you are interested in protein level changes (one or two proteins) and have antibodies, you should consider the LiCor.

Data Storage. A flash drive should be sufficient for data storage. Data can also be uploaded directly into the DRSC database for analysis and viewing with our heat map tool (plate view with analyzed quantity-based pseudocoloring).

Plate Type

Fluorescence read-out, read at the bottom: Black, clear bottom 384-well plates (Corning 3712; available at DRSC for bathing protocols only)

Fluorescence read-out, read at the top: White, solid bottom 384-well plates (Corning 3570).

Luminescence read-out: White, solid bottom 384-well plates (Corning 3570).

Note: plate types are included for your reference for optimization. We supply the plates at the DRSC (dsRNAs are supplied in the assay plate type you required).

Expert Tip: When switching from 96-well to 384-well format, some researchers notice a greater than the four-fold reduction in readout levels (i.e. more dramatic reduction in a luminescence signal than what would be expected simply based on the change in volume or cell number).