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DRSC
>> Screens
>> Publications from the DRSC
Publications from the DRSC (including methods, tools, off-target effects):
Wang J, Zhou X, Bradley PL, Chang SF,
Perrimon N, Wong ST. Cellular phenotype recognition for
high-content RNA interference genome-wide screening. J
Biomol Screen. 2008 Feb;13(1):29-39.
Ni JQ, Markstein M, Binari R, Pfeiffer B,
Liu LP, Villalta C, Booker M, Perkins L, Perrimon N. Vector
and parameters for targeted transgenic RNA interference in
Drosophila melanogaster. Nat Methods. 2008
Jan;5(1):49-51.
DasGupta R, Nybakken K, Booker M,
Mathey-Prevot B, Gonsalves F, Changkakoty B,Perrimon N. A
case study of the reproducibility of transcriptional
reporter cell-based RNAi screens in Drosophila. Genome
Biol. 2007;8(9):R203.
Ramadan N, Flockhart I, Booker M, Perrimon
N, Mathey-Prevot B. Design and implementation of
high-throughput RNAi screens in cultured Drosophila
cells. Nat Protoc. 2007;2(9):2245-64.
Perrimon N, Mathey-Prevot B. Applications of
high-throughput RNA interference screens to problems in cell
and developmental biology. Genetics. 2007
Jan;175(1):7-16.
Echeverri CJ, Beachy PA, Baum B, Boutros M,
Buchholz F, Chanda SK, Downward J, Ellenberg J, Fraser AG,
Hacohen N, Hahn WC, Jackson AL, Kiger A, Linsley PS, Lum L,
Ma Y, Mathey-Prévôt B, Root DE, Sabatini DM, Taipale J,
Perrimon N, Bernards R. Minimizing the risk of reporting
false positives in large-scale RNAi screens. Nat
Methods. 2006 Oct;3(10):777-9.
Kulkarni MM, Booker M, Silver SJ, Friedman
A, Hong P, Perrimon N, Mathey-Prevot B. Evidence of
off-target effects associated with long dsRNAs in Drosophila
melanogaster cell-based assays. Nat Methods. 2006
Oct;3(10):833-8.
Echeverri CJ, Perrimon N. High-throughput
RNAi screening in cultured cells: a user's guide. Nat Rev
Genet. 2006 May;7(5):373-84.
Flockhart I, Booker M, Kiger A, Boutros M,
Armknecht S, Ramadan N, Richardson K, Xu A, Perrimon N,
Mathey-Prevot B. FlyRNAi: the Drosophila RNAi screening
center database. Nucleic Acids Res. 2006 Jan
1;34(Database issue):D489-94
Armknecht S, Boutros M, Kiger A, Nybakken K,
Mathey-Prevot B, Perrimon N. High-throughput RNA
interference screens in Drosophila tissue culture
cells. Methods Enzymol. 2005;392:55-73.
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