Public Screens
Functional genomics reveals genes involved in protein
secretion and Golgi organization
Nature. 2006 Feb 2;439(7076):604-7.
Bard F,
Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama
H, Guizzunti G, Hu Y, Wendler F, Dasgupta R, Perrimon N,
Malhotra V
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A genome-wide RNA interference screen in Drosophila
melanogaster cells for new components of the Hh
signaling pathway
Nature Genetics 2005 Dec;37(12):1323-32. Epub 2005 Nov 20.
Kent Nybakken, Steven A Vokes, Ting-Yi Lin, Andrew P McMahon,
Norbert Perrimon
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Genome-wide RNAi analysis of JAK/STAT signaling components
in Drosophila
Genes and Development 2005 Aug 15;19(16):1861-70.
Epub 2005 Jul 29
Gyeong-Hun Baeg, Rui Zhou, Norbert Perrimon
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Genome-Wide RNAi Screen for Host Factors Required for
Intracellular Bacterial Infection
Science. 2005 Jul 14
Agaisse H, Burrack LS, Philips J, Rubin EJ, Perrimon N,
Higgins DE
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Drosophila RNAi Screen Reveals CD36 Family Member
Required for Mycobacterial Infection
Science. 2005 July 14
Philips JA, Rubin EJ, Perrimon N
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Genome-Wide RNAi Analysis of Growth and Viability in
Drosophila Cells
Science. 2004 Feb 6;303(5659):832-5
Michael
Boutros,
Amy Kiger,
Susan Armknecht, Kim Kerr, Marc Hild, Britta Koch, Stefan A. Haas,
Heidelberg Fly Array Consortium, Renato Paro,
Norbert Perrimon
|
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Completed Experiments Awaiting Analysis and Publication
Screen for mediators of doxorubicin induced apoptosis
| Experimenters: |
Dodzie Sogah |
| Description: |
This screen was designed to identify key genes
involved in regulating DNA damage induced cell death
in KC cells. Gene knockdown was performed for 48
hours, and then doxorubicin was added for an
additional 48 hours, after which cell viability was
accessed. |
| Screen Type: |
plate-reader-based assay |
| Contact: |
Dodzie Sogah
|
| Affiliation: |
Program of Biological and Biomedical Sciences,
Department of Cell Biology, Harvard Medical School. |
| Posted: | October 2004 |
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Cellular response to copper in Drosophila melanogaster
| Experimenters: |
Hasmik Yepiskoposyan |
| Description: |
The goal of this screen is to identify genes
involved in copper homeostasis and copper induced
transcriptional response. The metallothioneinA
(MtnA) gene is induced by the addition of copper. S2
cells with stably integrated reporter and reference
genes MtnA-Luciferase and tubulin-Renilla were
bathed in dsRNA and induced with copper. |
| Screen Type: |
plate-reader luminescence-based assay |
| Contact: |
Hasmik Yepiskoposyan
|
| Affiliation: |
Schaffner Lab; Institute of Molecular Biology,
University of Zurich |
| Posted: | October 2004 |
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Genome-wide screen for regulators of ERK/MAPK activation
| Experimenter: | Adam Friedman |
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| Description: |
Stimulation of ERK/MAPK activity in Drosophila
cells with multiple RTK ligands and screen for regulators by
dsRNA knockdown. |
| Screen Type: |
plate-reader, fluorescence-based assay |
| Contact: |
Adam Friedman,
Norbert Perrimon |
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School |
| Posted: | September 2004 |
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S2R+ morphology
| Experimenters: |
Buzz Baum, Amy Kiger, Frieder Schoeck |
| Description: |
S2R+ cells were grown for 5 days, replated prior and left
to adhere to plates after a few hours. Both the original
and replica plates were fixed and stained to visualize
Actin, Microtubules and DNA. |
| Screen Type: |
autoscope |
| Contact: |
Amy Kiger |
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School |
| Posted: | September 2004 |
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Genome-wide screen for host factors involved in Vesicular
Stomatitis virus replication
| Experimenters: |
Sara Cherry |
| Description: |
Infection of Drosophila cells with VSV and screen for host
factors by dsRNA knockdown. |
| Screen Type: |
plate-reader luminescence-based assay |
| Contact: |
Sara Cherry,
Norbert Perrimon,
Sean Whelan
|
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School
Whelan lab; Dept of Microbiology and Immunology, Harvard
Medical School |
| Posted: | September 2004 |
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Live Visual Screen of Fluorescent Labeled Primary Neurons
and Glia
| Experimenters: |
Katharine Sepp |
| Description: |
This broad screen looked for morphological changes
in fluorescently labeled primary neurons and glia
that were prepared from embryos. The screen
identified genes involved in neuronal and glial
outgrowth, differentiation, survival, axon
transport, and mature function. |
| Screen Type: |
visual |
| Contact: |
Katharine Sepp
|
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School
|
| Posted: | September 2004 |
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Genome-wide screen for regulators of Akt activation
| Experimenters: |
Lutz Kockel |
| Description: |
Stimulation of Akt phosphorylation in Drosophila cells
with Insulin and screen for regulators by dsRNA knockdown. |
| Screen Type: |
plate-reader fluorescence-based assay |
| Contact: |
Lutz Kockel,
Norbert Perrimon
|
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School |
| Posted: | September 2004 |
|---|
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Global RNAi screen for regulators of the
calcium-calcineurin-NFATsignalling pathway
| Experimenters: |
Yousang Gwack, Julie Nardone, Anjana Rao |
| Description: |
Our screen has two parts: the first is designed to
identify proteins whose knockdown results in
constitutitve nuclear localization of nuclear factor
of activated T-cells (NFAT) in resting cells, the
second proteins whose knockdown prevents NFAT
localization to the nucleus in cells activated by
chemically-induced calcium influx. In the first
screen we anticipate finding proteins involved in
cytoplasmic sequestration of NFAT in resting cells,
those involved in maintaining intracellular calcium
and calcineurin activity at low levels, and those
responsible for exporting NFAT from the nucleus. In
the second screen we expect to identify candidates
involved in increasing [Ca2+]i levels and calcineurin
activity, or facilitating the nuclear import of
NFAT. |
| Screen Type: |
visual screen |
| Contact: |
Anjana
Rao, Ph.D.
|
| Affiliation: |
CBR Institute for Biomedical Research
HMS Department of Pathology
220 Longwood Avenue
Boston MA 02115 |
| Posted: | September 2004 |
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Screening for novel components of the Notch signaling
pathway
| Experimenters: |
Brian DeDecker |
| Description: |
Drosophila Kc cells were incubated with the
library dsRNAs for four days. On the fourth day the
cells were split into 6 daughter plates containing
duplicates of 3 different firefly luciferase
reporter constructs. The first used a viral promoter
that acted as a control for cell viability and
general transcription activity. The next experiment
used the entire Notch specific m3 promoter to
measure the base line effect of the dsRNA. While
the final experiment contained the same m3 promoter
construct cotransfected with constitutively active
Notch to measure any Notch specific effects. All
three experiments were analyzed as a complete self
controlled set. |
| Screen Type: |
plate-reader |
| Contact: |
Brian DeDecker
|
| Affiliation: |
Artavanis-Tsakonas Lab; Department of Cell Biology,
Harvard Medical School |
| Posted: | September 2004 |
|---|
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Regulation of the G1/S transition and the Rb-E2F pathway by
a genome-wide RNAi analysis
| Experimenters: |
Jianrong Lu |
| Description: |
The G1/S phase transition is a crucial point in
cell cycle control commonly altered in human cancer,
and is regulated by the cyclin-cdk-Rb-E2F
pathway. The present screen is aimed at
systematically defining genes involved in this
regulatory mechanism. |
| Screen Type: |
plate-reader |
| Contact: |
Jianrong Lu,
Philip Leder (PI)
|
| Affiliation: |
Leder Lab; Department of Genetics, Harvard Medical
School |
| Posted: | September 2004 |
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A functional genomic screen to identify novel clock genes
involved in the circadian light entrainment pathway
| Experimenters: |
Sriram Sathyanarayanan |
| Description: |
In Drosophila circadian clock, light
information is transmitted to the clock by a
circadian photoreceptor, Cryptochrome (CRY). In
response to light, CRY binds to a circadian core
clock component, TIMELESS (TIM) and both proteins
are targeted for degradation. Light dependent
degradation of CRY can be effectively recapitulated
in cultured Drosophila S2 cells. To identify novel
components involved in this pathway we developed an
assay for determining CRY stability in living cells
using a CRY-luciferase (LUC) fusion protein. In
response to light treatment the CRY-LUC fusion
protein, like CRY, is degraded. Cells carrying
CRY-luc were treated with ds-RNA and assayed for
luciferase activity in the dark and in the presence
of light. Actin-renilla luciferase levels were
measured as an internal control. Genes that appear
to be required for light-dependent CRY degradation
are currently being analyzed. |
| Screen Type: |
plate reader-luminescence |
| Contact: |
Sriram Sathyanarayanan,
Amita Sehgal
|
| Affiliation: |
Sehgal Lab
Dept. of Neuroscience
HHMI/University of Pennsylvania, STM 232
36th and Hamilton Walk
Philadelphia, PA 19104 |
| Posted: | March 2005 |
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Positive loop of the molecular circadian clock in
Drosophila
| Experimenters: |
Jerome Menet |
| Description: |
This screen is designed to identify genes involved in
the generation of circadian rhythms in Drosophila. This
screen uses a S2 cell line that expresses a luciferase
reporter gene that can be activated by the 2 majors
transcription factors of the molecular circadian clock,
CLOCK and CYCLE. Cells are incubated with dsRNA for 4
days, and then firefly luciferase activity is measured
and normalized to Renilla luciferase activity. |
| Screen Type: |
Luminescence-based reporter assay |
| Contact: |
Jerome Menet,
Michael Rosbash
|
| Affiliation: |
Rosbash
Lab, HHMI/Department of Biology
MS008
Brandeis University
415 South Street
Waltham, MA 02454 |
| Posted: | March 2005 |
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Chlamydia interaction with host cells
| Experimenters: |
Isabelle Derre |
| Description: |
Genome-wide screen for host factors important for
entry, survival and multiplication of Chlamydia caviae.
The assay is based on the detection of increased or
decreased intracellular growth by fluorescence microscopy.
The bacteria are detected using a Chlamydia-specific FITC
conjugated antibody. |
| Screen Type: |
visual |
| Contact: |
Isabelle Derre
|
| Affiliation: |
Section of Microbial Pathogenesis
Yale University School of Medicine
New Haven, CT
USA |
| Posted: | March 2005 |
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Identification of genes required for HOW-dependent RNA
degradation
| Experimenters: |
Ronit Nir, Talila Volk |
| Description: |
The Drosophila Held Out Wing (HOW) protein
controls different aspects of tissue
differentiation in tendon cells and in the
mesoderm. One of its two isoforms, HOW(L), binds
to the 3'UTR of its mRNA targets, including
the transcription factor stripe, and induces their
degradation. In this genome-scale screen, we try
to shed light on the mechanism of HOW(L) action,
through identification of factors that are
involved in this process. To this end, we
co-transfected S2R+ cells with HOW(L) and a
reporter gfp mRNA fused to stripe 3'UTR, which is
down-regulated by HOW(L). Following 4 days
incubation with the dsRNA collection, the GFP
levels were quantified by a plate reader, and
genes that caused significant changes in GFP
levels were selected. We are now in the process of
selecting genes and performing additional assays
to determine their specificity to HOW(L)
activity. |
| Screen Type: |
plate reader-fluorescence |
| Contact: |
Ronit Nir
|
| Affiliation: |
Volk Lab, Department of Molecular Genetics
Weizmann Institute of Science
Rehovot 76100
Israel |
| Posted: | March 2005 |
|---|
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Genes required for the HIF-dependent transcriptional
response to hypoxia.
| Experimenters: |
Andrés Dekanty |
| Description: |
A reporter in which a HIF-Responsive-Element (HRE)
drives the expression of firefly luciferase was
stably transfected in S2 cells. Expression of the
reporter is strongly induced in hypoxia or upon
exposure to the iron chelating agent, Deferroxiamine
(DFO). The screen led to the identification of genes
that abrogate DFO-dependent induction of the
HRE-luciferase reporter. |
| Screen Type: |
plate reader-luminescence |
| Contact: |
Pablo Wappner
|
| Affiliation: |
Wappner Lab, Instituto Leloir
Patricias Argentinas 435
Buenos Aires (1405)
República Argentina |
| Posted: | March 2005 |
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Nuclear targeting factor of Mad
| Experimenter(s): |
Lan Xu |
| Description: |
Search for genes that are required for Dpp-induced
nuclear accumulation of Mad |
| Screen Type: |
visual |
| Contact: |
Lan Xu
|
| Affiliation: |
Program in Molecular Medicine,
Univ. of Massachusetts Medical School |
| Posted: | November 2005 |
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Genetic modifiers of cell survival following exposure to
DNA damaging agents
| Experimenter(s): |
Alex Bishop |
| Description: |
A series of related full-genome screens:
- Examine genes required for viability
- Examine genes required for viability following
exposure to sublethal exposure to Methyl Methane
Sulphonate
- Examine genes required for viability following
exposure to sublethal exposure to UV
- Examine genes required for viability following
exposure to sublethal exposure to Bleomycin
- Examine genes required for viability following
exposure to sublethal exposure to Cisplatin
- Examine genes required for viability following
exposure to sublethal exposure to Etoposide
|
| Screen Type: |
plate reader |
| Contact: |
Alex
Bishop
|
| Affiliation: |
Childrens Cancer Research Institute,
University of Texas Health Science Center at
San Antonio |
| Posted: | November 2005 |
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p53 Viability
| Experimenter(s): |
Alex Bishop |
| Description: |
A series of related full-genome screens:
- Examine genes required for viability in the absence
of p53
- Examine genes required for viability following
exposure to sublethal exposure to Methyl Methane
Sulphonate in the absence of p53
- Examine genes required for viability following
exposure to sublethal exposure to UV in the absence
of p53
- Examine genes required for viability following
exposure to sublethal exposure to Bleomycin in the
absence of p53
- Examine genes required for viability following
exposure to sublethal exposure to Cisplatin in the
absence of p53
- Examine genes required for viability following
exposure to sublethal exposure to Etoposide in the
absence of p53
|
| Screen Type: |
plate reader |
| Contact: |
Alex
Bishop
|
| Affiliation: |
Childrens Cancer Research Institute,
University of Texas Health Science Center at
San Antonio |
| Posted: | November 2005 |
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Global RNAi screen for regulators of the
calcium-calcineurin-NFATsignalling pathway - Follow-up
| Experimenter(s): |
Yousang Gwack, Sonal Srikanth, Anjana Rao |
| Description: |
Global RNAi screen for regulators of the
calcium-calcineurin-NFATsignalling pathway |
| Screen Type: |
visual, localization change of target proteins |
| Contact: |
Yousang
Gwack
|
| Affiliation: |
CBR Institute for Biomedical Research
HMS Department of Pathology
220 Longwood Avenue
Boston MA 02115 |
| Posted: | November 2005 |
|---|
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Genome-wide RNAi screen to identify yet unknown proteins
involved in mitochondrial dynamics.
| Experimenter(s): |
Shilpa Gandre |
| Description: |
Mitochondria are highly dynamic organelles in the
cell, constantly dividing and fusing. This screen used
flat, adherent S2R+ cells. Mitochondria were
visualized at the end of three days RNAi treatment,
with Mitotracker Red a vital fluorescence dye. Cells
were fixed and screened manually using a Zeiss
axiovert microscope in our lab since the red
fluorescence was too weak to be meaningfully detected
by the autoscope. The gross changes in mitochondrial
distribution and morphology were noted. |
| Screen Type: |
Visual |
| Contact: |
Shilpa
Gandre and Alexander van der Bliek
|
| Affiliation: |
Alexander van der Bliek
Department of Biological Chemistry
David Geffen School of Medicine,
650,S.Charles Young Drive, 33-257 CHS
University of California at Los Angeles, CA 90095 |
| Posted: | November 2005 |
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Genome-wide RNAi screen for genes required for phagocytosis
of apoptotic cells in S2 cells
| Experimenter(s): |
Nathalie Franc, Leigh Cuttell |
| Description: |
Genome-wide RNAi screen for genes required for
phagocytosis of apoptotic cells in S2 cells |
| Screen Type: |
visual |
| Contact: |
Nathalie
Franc (PI)
|
| Affiliation: |
Franc Lab at Medical Research Council Laboratory for
Molecular Cell Biology & Cell Biology Unit |
| Posted: | November 2005 |
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Regulators of X-chromosome dosage compensation
| Experimenter(s): |
Erica Larschan, Mitzi Kuroda |
| Description: |
Identification of new factors involved in targeting the
MSL (Male Specific Letha) complex to the X chromosome |
| Screen Type: |
Visual Screen |
| Contact: |
Erica Larschan
|
| Affiliation: |
Kuroda Lab, Brigham and Women's Hospital |
| Posted: | November 2005 |
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Genes involved in store-operated calcium entry
| Experimenter(s): |
Shenyuan Zhang |
| Screen Type: |
plate reader |
| Contact: |
Shenyuan
Zhang
Michael
Cahalan
|
| Affiliation: |
Michael Cahalan's lab at Univ. of California,
Irvine |
| Posted: | November 2005 |
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Host Factors required for Sindbis replication
| Experimenter(s): |
Sara Cherry |
| Screen Type: |
autoscope |
| Contact: |
Sara Cherry,
Norbert Perrimon
|
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School |
|
| Posted: | March 2006 |
|---|
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Genome-Wide Screen for Genes Involved in the Secretion and
Release of Cholesterol-Modified Hedgehog
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Genome wide RNAi Screen for molecules linking G
protein-coupled receptors and small GTPases to gene
expression in Drosophila melanogaster cultured cells.
| Experimenter(s): |
Robert Dorsam |
| Screen Type: |
plate reader |
| Contact: |
Robert Dorsam
|
| Affiliation: |
Gutkind Lab
NATIONAL INSTITUTES OF HEALTH/NIDCR
BUILDING 30 ROOM 211
30 CONVENT DR MSC 4340
BETHESDA MD 20892-4340 |
| Posted: | March 2006 |
|---|
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Genome-wide RNAi screen for modifiers of intracellular
aggregates derived from mis-folded proteins
| Experimenter(s): |
Sheng Zhang |
| Screen Type: |
autoscope |
| Contact: |
Sheng Zhang
|
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School |
|
| Posted: | March 2006 |
|---|
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Genome-wide RNAi screen to identify essential factors for
nuclear mRNA export in Drosophila
| Experimenter(s): |
Natalie Gilks |
| Screen Type: |
autoscope |
| Contact: |
Natalie Gilks
|
| Affiliation: |
Silver Lab
Harvard Medical School
Systems Biology Alpert 536
200 Longwood Ave
Boston, MA 02115 |
| Posted: | March 2006 |
|---|
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Signal transduction pathways leading to mast cell
degranultion and allergy
| Experimenter(s): |
Monika Vig |
| Screen Type: |
plate reader |
| Contact: |
Monika Vig
|
| Affiliation: |
Kinet Lab
Beth Israel Deaconess Med Ctr
Experimental Pathology-Rm 227 C
99 Brookline Ave
Boston, MA 02215 |
| Posted: | March 2006 |
|---|
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Genes regulating lipid storage in Drosophila melanogaster
| Experimenter(s): |
Mathias Beller |
| Screen Type: |
autoscope |
| Contact: |
Mathias Beller
|
| Affiliation: |
Oliver Lab |
| Posted: | March 2006 |
|---|
|
Genes required for clustering supernumerary centrosomes
| Experimenter(s): |
Mijung Kwon |
| Screen Type: |
autoscope |
| Contact: |
Mijung Kwon
|
| Affiliation: |
Pellman Lab
Dana Farber Cancer Institute
M 621a
44 Binney St
Boston, MA 02115 |
| Posted: | March 2006 |
|---|
|
Identification of Proteins Required for Histone mRNA
Biosynthesis
|
Identification of host factors required for Flock house
virus replication
| Experimenter(s): |
Sara Cherry and Lin Hao |
| Screen Type: |
autoscope |
| Contact: |
Sara Cherry
|
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School |
|
| Posted: | March 2006 |
|---|
|
PVR modifier screen
| Experimenter(s): |
Katja Brückner |
| Screen Type: |
autoscope |
| Contact: |
Katja Brückner
|
| Affiliation: |
Perrimon Lab; Department of Genetics, Harvard Medical
School |
| Posted: | March 2006 |
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